MTHFD1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38574
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human MTHFD1 (NP_005947.3).
Sequence:
MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITP
Sequence:
MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSNLYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQLPLDSENSINTEEVINAIAPEKDVDGLTSINAGKLARGDLNDCFIPCTPKGCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTAHLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGRKVVGDVAYDEAKERASFITP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MTHFD1 Antibody
Western Blot: MTHFD1 Antibody [NBP3-38574] -
Western Blot: MTHFD1 Antibody [NBP3-38574] - Western blot analysis of various lysates using MTHFD1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: MTHFD1 Antibody [NBP3-38574] -
Immunocytochemistry/ Immunofluorescence: MTHFD1 Antibody [NBP3-38574] - Immunofluorescence analysis of U-2 OS cells using MTHFD1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for MTHFD1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:200 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MTHFD1
Alternate Names
C1-THF synthase, EC 6.3.4.3, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, MTHFCC-1-tetrahydrofolate synthase, cytoplasmic, MTHFDcytoplasmic C-1-tetrahydrofolate synthase
Gene Symbol
MTHFD1
Additional MTHFD1 Products
Product Documents for MTHFD1 Antibody
Product Specific Notices for MTHFD1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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