MTHFD2 Antibody (4G7-2G3)

Novus Biologicals, part of Bio-Techne | Catalog # H00010797-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G7-2G3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MTHFD2 Antibodies »

Product Specifications

Immunogen

MTHFD2 (AAH01548, 16 a.a. ~ 344 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLRLRPFHLAAVRNEAVVISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINSETIMKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFHVINVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEREVLKSKELGVATN

Reactivity Notes

Other species not tested.

Localization

Mitochondrial

Specificity

MTHFD2 - methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MTHFD2 Antibody (4G7-2G3)

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01]

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01]

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01] - MTHFD2 monoclonal antibody (M01), clone 4G7-2G3 Analysis of MTHFD2 expression in HepG2.
Immunohistochemistry-Paraffin: MTHFD2 Antibody (4G7-2G3) [H00010797-M01]

Immunohistochemistry-Paraffin: MTHFD2 Antibody (4G7-2G3) [H00010797-M01]

Immunohistochemistry-Paraffin: MTHFD2 Antibody (4G7-2G3) [H00010797-M01] - Analysis of monoclonal antibody to MTHFD2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 3 ug/ml.
Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01]

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01]

Western Blot: MTHFD2 Antibody (4G7-2G3) [H00010797-M01] - Analysis of MTHFD2 expression in transfected 293T cell line by MTHFD2 monoclonal antibody (M01), clone 4G7-2G3.Lane 1: MTHFD2 transfected lysate(26.8 KDa).Lane 2: Non-transfected lysate.

Applications for MTHFD2 Antibody (4G7-2G3)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MTHFD2

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in multiple transcripts encoding different isoforms. This gene has a pseudogene on chromosome 7.

Alternate Names

bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, mitochondrial, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase, NMDMC

Entrez Gene IDs

10797 (Human)

Gene Symbol

MTHFD2

OMIM

604887 (Human)

UniProt

AAH01548 (Human)

Additional MTHFD2 Products

Product Documents for MTHFD2 Antibody (4G7-2G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MTHFD2 Antibody (4G7-2G3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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