MTHFR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21401

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MTHFR Antibodies »

Product Summary for MTHFR Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPI

Reactivity Notes

Mouse (88%), Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MTHFR Antibody

Western Blot: MTHFR Antibody [NBP3-21401] -

Western Blot: MTHFR Antibody [NBP3-21401] -

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-21401] -

Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-21401] -

Analysis in human epididymis and placenta tissues using Anti-MTHFR antibody. Corresponding MTHFR RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-21401] -

Immunohistochemistry-Paraffin: MTHFR Antibody [NBP3-21401] -

Staining of human pituitary gland shows strong positivity in most anterior cells.
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Applications for MTHFR Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTHFR

Methylenetetrahydrofolate reductase (EC 1.5.1.20) catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a cosubstrate for homocysteine remethylation to methionine.[supplied by OMIM]

Alternate Names

EC 1.5.1.20, methylenetetrahydrofolate reductase, methylenetetrahydrofolate reductase (NAD(P)H)5,10-methylenetetrahydrofolate reductase (NADPH)

Gene Symbol

MTHFR

Additional MTHFR Products

Product Documents for MTHFR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MTHFR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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