MUC1 Antibody (MUC1/967) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47885
![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]](https://resources.bio-techne.com/images/products/MUC1-Antibody-MUC1-967-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47885-img0002.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]")
![Western Blot: MUC1 Antibody (MUC1/967)Azide and BSA Free [NBP2-47885]](https://resources.bio-techne.com/images/products/MUC1-Antibody-MUC1-967-Azide-and-BSA-Free-Western-Blot-NBP2-47885-img0004.jpg "Western Blot: MUC1 Antibody (MUC1/967)Azide and BSA Free [NBP2-47885]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MUC1/967
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human milk-fat globule membranes (HMFGM) (Uniprot: P15941 )
Epitope
APDTR
Reactivity Notes
Others not known.
Localization
Cytoplasmic and cell surface
Specificity
This monoclonal antibody recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein. The dominant epitope of this monoclonal antibody is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the monoclonal antibody. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This monoclonal antibody reacts with both normal and malignant epithelia of various tissues including breast and colon.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44659).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MUC1 Antibody (MUC1/967) - Azide and BSA Free
Western Blot: MUC1 Antibody (MUC1/967)Azide and BSA Free [NBP2-47885]
Western Blot: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885] - Western Blot Analysis of human MCF-7 cell lysate using MUC1 antibody (MUC1/967).![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]](https://resources.bio-techne.com/images/products/MUC1-Antibody-MUC1-967-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47885-img0003.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]")
Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]
Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885] - Human Endometrial Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).![Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]](https://resources.bio-techne.com/images/products/MUC1-Antibody-MUC1-967-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47885-img0001.jpg "Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]")
Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885]
Immunohistochemistry-Paraffin: MUC1 Antibody (MUC1/967) - Azide and BSA Free [NBP2-47885] - Human Ovarian Carcinoma stained with EMA Monoclonal Antibody (MUC1/967).Applications for MUC1 Antibody (MUC1/967) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistology (Formalin-paraffin): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MUC-1
Overexpression of mucins, including MUC1, is a feature of many epithelial cancers (1,3,5,6). The presence of truncated glycan structures called tumor-associated carbohydrate antigens (TACAs) on MUC1 play a role in cancer progression and a loss of apical-basal polarity (5). Carbohydrate-binding partners called lectins are the primary binding partners of TACAs that give rise to the pro-tumor microenvironment and metastasis (5). Given this unique feature, TACAs are a potential target for cancer immunotherapies (5). There are a number of vaccines, drugs, and antibodies targeting MUC1 for treatment of a variety of cancers including breast, lung, and prostate (6). In addition to a role in cancer progression, MUC1, and specifically the CT portion, has been shown to have a positive, anti-inflammatory role in a variety of lung and airway infections (7).
References
1. Khodabakhsh, F., Merikhian, P., Eisavand, M. R., & Farahmand, L. (2021). Crosstalk between MUC1 and VEGF in angiogenesis and metastasis: a review highlighting roles of the MUC1 with an emphasis on metastatic and angiogenic signaling. Cancer cell international. https://doi.org/10.1186/s12935-021-01899-8
2. Nath, S., & Mukherjee, P. (2014). MUC1: a multifaceted oncoprotein with a key role in cancer progression. Trends in molecular medicine. https://doi.org/10.1016/j.molmed.2014.02.007
3. Dhar, P., & McAuley, J. (2019). The Role of the Cell Surface Mucin MUC1 as a Barrier to Infection and Regulator of Inflammation. Frontiers in cellular and infection microbiology. https://doi.org/10.3389/fcimb.2019.00117
4. Uniprot (P15941)
5. Beckwith, D. M., & Cudic, M. (2020). Tumor-associated O-glycans of MUC1: Carriers of the glyco-code and targets for cancer vaccine design. Seminars in immunology. https://doi.org/10.1016/j.smim.2020.101389
6. Almasmoum H. (2021). The Roles of Transmembrane Mucins Located on Chromosome 7q22.1 in Colorectal Cancer. Cancer management and research. https://doi.org/10.2147/CMAR.S299089
7. Ballester, B., Milara, J., & Cortijo, J. (2021). The role of mucin 1 in respiratory diseases. European respiratory review : an official journal of the European Respiratory Society. https://doi.org/10.1183/16000617.0149-2020
Long Name
Mucin 1, Cell Surface-associated
Alternate Names
CD227, Episialin, H23AG, KL-6, Mucin-1, PEM, PEMT
Gene Symbol
MUC1
Additional MUC-1 Products
Product Documents for MUC1 Antibody (MUC1/967) - Azide and BSA Free
Product Specific Notices for MUC1 Antibody (MUC1/967) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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