MUC4 Antibody (5B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00004585-M07

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5B12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MUC-4 Antibodies »

Product Specifications

Immunogen

MUC4 (NP_004523, 79 a.a. ~ 188 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVSLFPYGADAGDLEFVRRTVDFTSPLFKPATGFPLGSSLRDSLYFTDNGQIIFPESDYQIFSYPNPLPTGFTGRDPVALVAPFWDDADFSTGRGTTFYQEYETFYGEHS

Specificity

MUC4 - mucin 4, tracheobronchial

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MUC4 Antibody (5B12)

Western Blot: MUC4 Antibody (5B12) [H00004585-M07]

Western Blot: MUC4 Antibody (5B12) [H00004585-M07]

Western Blot: MUC4 Antibody (5B12) [H00004585-M07] - Analysis of MUC4 expression in HeLa (Cat # L013V1).
Immunohistochemistry-Paraffin: MUC4 Antibody (5B12) [H00004585-M07]

Immunohistochemistry-Paraffin: MUC4 Antibody (5B12) [H00004585-M07]

Immunohistochemistry-Paraffin: MUC4 Antibody (5B12) [H00004585-M07] - Analysis of monoclonal antibody to MUC4 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml
ELISA: MUC4 Antibody (5B12) [H00004585-M07]

ELISA: MUC4 Antibody (5B12) [H00004585-M07]

ELISA: MUC4 Antibody (5B12) [H00004585-M07] - Detection limit for recombinant GST tagged MUC4 is approximately 0.3ng/ml as a capture antibody.

Applications for MUC4 Antibody (5B12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MUC-4

The major constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, are highly glycosylated proteins called mucins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. This gene encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of this gene have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. This gene contains a region in the coding sequence which has a variable number (>100) of 48 nt tandem repeats. [provided by RefSeq]

Long Name

Mucin 4, Cell Surface-associated

Alternate Names

ASGP, MUC4

Entrez Gene IDs

4585 (Human)

Gene Symbol

MUC4

OMIM

158372 (Human)

UniProt

NP_004523 (Human)

Additional MUC-4 Products

Product Documents for MUC4 Antibody (5B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MUC4 Antibody (5B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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