MUL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31363

Novus Biologicals, part of Bio-Techne
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NBP2-31363-0.025mg
NBP2-31363-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MUL1 Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to a C-terminal portion of the human MUL1 protein (between residues 200-352) [UniProt Q969V5]

Reactivity Notes

The immunogen sequence shows 99% similarity to Monkey's MUL1 and is 84% similar to isoform 1 and 3 of Mouse's MUL1 .

Localization

Mitochondria, Peroxisomes

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MUL1 Antibody

Western Blot: MUL1 Antibody [NBP2-31363]

Western Blot: MUL1 Antibody [NBP2-31363]

Western Blot: MUL1 Antibody [NBP2-31363] - Western blot analysis of K562 (A), Rt4 (B), and SH-SY5Y (C) cell lysate using MUL1 antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: MUL1 Antibody [NBP2-31363]

Immunocytochemistry/ Immunofluorescence: MUL1 Antibody [NBP2-31363]

Immunocytochemistry/Immunofluorescence: MUL1 Antibody [NBP2-31363] - MUL1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody concentration of 0.1 ug/ml was used.
Western Blot: MUL1 Antibody [NBP2-31363]

Western Blot: MUL1 Antibody [NBP2-31363]

Western Blot: MUL1 Antibody [NBP2-31363] - WB analysis of MUL1 protein in lysate of human liver and partial recombinant MUL1 protein by using the primary antibody concentration of 2ug/ml and 0.5 ug/ml respectively.

Applications for MUL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.1-2 ug/ml

Western Blot

2 ug/ml
Application Notes
MUL1 (mitochondrial ubiquitin ligase activator of NFKB 1) is a 352 amino acids long protein (predicted molecular weight 39.8 kDa) which localizes to the mitochondrion's outer membrane as a multi-pass membrane protein and from there, it may get transported to the peroxisomes via mitochondrion-derived vesicles. In our Western blot validation, this MUL1 antibody detected a specific target band at ~40 kDa in human liver lysate.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

No buffer

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MUL1

MUL1 is an E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, MUL1 activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Alternate Names

C1orf166mitochondrial E3 ubiquitin ligase 1, E3 ubiquitin-protein ligase MUL1, EC 6.3.2, EC 6.3.2.-, FLJ12875, GIDERP11-401M16.2, Growth inhibition and death E3 ligase, MAPLE3 ubiquitin ligase, mitochondrial E3 ubiquitin protein ligase 1, mitochondrial ubiquitin ligase activator of NF-kB, mitochondrial ubiquitin ligase activator of NFKB 1, Mitochondrial-anchored protein ligase, MULANchromosome 1 open reading frame 166, Putative NF-kappa-B-activating protein 266, RING finger protein 218, RNF218mitochondria-anchored protein ligase

Entrez Gene IDs

79594 (Human)

Gene Symbol

MUL1

Additional MUL1 Products

Product Documents for MUL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MUL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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