MUPP1 Antibody

Western Blot: MUPP1 Antibody [NBP1-49975]

Western Blot: MUPP1 Antibody [NBP1-49975] - Detection of Human MUPP1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MUPP1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. MUPP1 was also immunoprecipitated by rabbit anti-MUPP1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated MUPP1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Western Blot: MUPP1 Antibody [NBP1-49975] -

Western Blot: MUPP1 Antibody [NBP1-49975] - LDOC1 interacts with Ligand Of Numb-Protein X 1 (LNX1) to promote phospho-JAK2 (pJAK2) degradation through the ubiquitin–proteasome pathway. (A,B) LDOC1 enhances proteasomal degradation (A) & ubiquitination (B) of pJAK2 in basal & IL-6-stimulated A549-derived cell lines. A549-derived cell lines were starved overnight & pretreated with CHX (100 μg/mL), either alone or with proteasome inhibitor MG132 (20 uM) for 3 h before IL-6 (100 ng/mL) stimulation. The cells were lysed in an NP-40 lysis buffer containing MG132 (20 μg/mL) & ubiquitin aldehyde (20 μg/mL) to inhibit isopeptidase activities. (A) Western blotting was performed to examine the levels of indicated proteins. (B,C) The cell extracts were precleared with mouse or rabbit IgG (IgGm or IgGr, respectively) before being immunoprecipitated using antibodies against either pJAK2 (B,C) or against LDOC1 & LNX1 antibodies (C). The input & the Immunoprecipitation (IP) fractions were analyzed through immunoblotting (blot) using antibodies that recognized the indicated proteins. After being stripped, the membrane was reblotted with an anti-pJAK2 antibody (B). (D) Associations between LDOC1-LNX1 & LDOC1-pJAK2 in IL-6-treated A549 & A549-shCtrl-D10 cells. IL-6 stimulated cells were subjected to immunofluorescence assay (IFA) by using anti-LDOC1-DyLight594 (red), anti-LNX1-FITC (green), & anti-pJAK2-FITC (green), respectively. The 4′,6-diamidino-2-phenylindole (DAPI, blue) was used for nuclear counterstaining. Images were captured using a confocal microscope. IP or IFA performed with IgGm or IgGr were used as negative controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30634502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.