MuRF1/TRIM63 Antibody (6G6)

Novus Biologicals, part of Bio-Techne | Catalog # H00084676-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6G6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MuRF1/TRIM63 Antibodies »

Product Specifications

Immunogen

TRIM63 (NP_115977, 254 a.a. ~ 352 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGH

Reactivity Notes

Human. Other species not tested.

Specificity

TRIM63 - tripartite motif-containing 63

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MuRF1/TRIM63 Antibody (6G6)

Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01]

Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01]

Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 expression in transfected 293T cell line by TRIM63 monoclonal antibody (M01), clone 6G6. Lane 1: TRIM63 transfected lysate(40.2 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01]

Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01]

Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of monoclonal antibody to TRIM63 on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01]

Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01]

Western Blot: MuRF1/TRIM63 Antibody (6G6) [H00084676-M01] - Analysis of TRIM63 over-expressed 293 cell line, cotransfected with TRIM63 Validated Chimera RNAi ( Cat # H00084676-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM63 monoclonal antibody (M01), clone 6G6 (Cat # H00084676-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for MuRF1/TRIM63 Antibody (6G6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA and immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MuRF1/TRIM63

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Long Name

Tripartite Motif-containing 63

Alternate Names

IRF, RNF28, SMRZ, TRIM63

Entrez Gene IDs

84676 (Human)

Gene Symbol

TRIM63

OMIM

606131 (Human)

UniProt

NP_115977 (Human)

Additional MuRF1/TRIM63 Products

Product Documents for MuRF1/TRIM63 Antibody (6G6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MuRF1/TRIM63 Antibody (6G6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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