Key Product Details

Species Reactivity

Validated:

Virus

Cited:

Virus

Applications

Validated:

Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2C6.H1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Murine Hepatitis Virus A59 nsp9 Antibodies »

Product Specifications

Immunogen

This Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) was produced in mice by repeated immunizations with E.coli derived full-length Murine Hepatitis Virus A59 nsp9 protein. This protein is part of the viral replicase polyprotein. (Uniprot: P0C6V0)

Reactivity Notes

This antibody is directed against the Murine Hepatitis Virus A59 nsp9 protein. No cross reactivity occurs with SARS CoV nsp9. Cross reactivity with homologues from other sources has not been tested.

Specificity

No cross reactivity occurs with SARS CoV nsp9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This product was purified from tissue culture supernatant fluid by Protein A chromatography

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1)

Immunocytochemistry/ Immunofluorescence: Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) [NBP2-21671]

Immunocytochemistry/ Immunofluorescence: Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) [NBP2-21671]

Immunocytochemistry/Immunofluorescence: Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1) [NBP2-21671] - 6-h post infection in mouse L cells. Cells were fixed in 3% para-formaldehyde. For detection Cy2 conjugated Goat-anti-Mouse IgG MX10 was used.
Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1)

Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1)

Western blotting using a nsp9 antibody to detect protein in various lysates, 6h post MHV infection. Lane 1 shows no cross-reactivity with Sected Vero cells. Negative controls (lanes 2 and 4) show no staining. Specific reactivity against MHV-A59 nsp9 from infected mouse L cells is shown in lane 3. Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.

Applications for Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:1000
Application Notes
This antibody has been tested for use in immunofluorescence microscopy and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells were grown on glass slides followed by infection with MHV-A59 strain and fixation with PBS/3% PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Murine Hepatitis Virus A59 nsp9

Alternate Names

2'-O-MT, 3' to 5' exonuclease, 3C-like proteinase, HEL1, leader protein, NendoU, nsp10, nsp11, NSP2, nsp4, nsp6, nsp7, nsp8, nsp9, ORF1a polyprotein, ORF1ab polyprotein, PLpro, polyprotein pp1a, polyprotein pp1ab, RNA-dependent RNA polymerase

Gene Symbol

ORF1ab

UniProt

P0C6V0 (Virus)

Additional Murine Hepatitis Virus A59 nsp9 Products

Product Documents for Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Murine Hepatitis Virus A59 nsp9 Antibody (2C6.H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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