Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94511
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 251-350 of human Muscarinic Acetylcholine Receptor M1/CHRM1 (NP_000729.2). SPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 AntibodyAzide and BSA Free [NBP2-94511]
Western Blot: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of extracts of various cell lines, using CHRM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.Immunocytochemistry/ Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody - Azide and BSA Free [NBP2-94511]
Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Analysis of SH-SY5Y cells using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear stainingImmunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody - Azide and BSA Free [NBP2-94511]
Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M1/CHRM1 Antibody [NBP2-94511] - Mouse brain using CHRM1 Rabbit pAb at dilution of 1:200 (40x lens).Applications
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:500 - 1:2000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CHRM1
Long Name
Cholinergic Receptor Muscarinic 1
Alternate Names
HM1, M1, M1R, mAChR M1
Gene Symbol
CHRM1
Additional CHRM1 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov