Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2805

Novus Biologicals, part of Bio-Techne
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NB120-2805
NB120-2805-125ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 31-1D1

Concentration

2 mg/ml

View all CHRM2 Antibodies »

Product Specifications

Immunogen

Purified porcine cardiac m2 muscarinic acetylcholine receptor.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Plasma membrane

Specificity

This is specific for the m2 mAChR subtype.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805]

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805]

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1) [NB120-2805] - Human kidney tissue.

Applications for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:20

Immunoprecipitation

1:10 - 1:500

Western Blot

1:1000
Application Notes
WB: Detects 60 - 80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR / CHRM2.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHRM2

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus.

Long Name

Cholinergic Receptor Muscarinic 2

Alternate Names

HM2, mAChR M2

Entrez Gene IDs

1129 (Human); 243764 (Mouse); 81645 (Rat)

Gene Symbol

CHRM2

Additional CHRM2 Products

Product Documents for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (31-1D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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