Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26152

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all CHRM2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QNGDEKQNIVARK corresponding to internal region according to NP_000730.1.

Specificity

Reported variants represent identical protein: NP_001006628.1, NP_001006633.1, NP_001006627.1, NP_000730.1, NP_001006629.1, NP_001006630.1, NP_001006631.1, NP_001006632.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody

Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NBP2-26152]

Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NBP2-26152]

Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NBP2-26152] - NBP2-26152 (2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in mouse brain lysates (calculated MW of 51.5 kDa band according to mouse NP_987076.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHRM2

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus.

Long Name

Cholinergic Receptor Muscarinic 2

Alternate Names

HM2, mAChR M2

Entrez Gene IDs

1129 (Human); 243764 (Mouse)

Gene Symbol

CHRM2

Additional CHRM2 Products

Product Documents for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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