Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15165

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-15165
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CHRM3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3/CHRM3. UniProt ID (P20309).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)

Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]

Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]

Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165] - Positive WB detected in:PC3 whole cell lysate. All lanes: Muscarinic Acetylcholine Receptor M3/CHRM3 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 67 KDa. Observed band size: 67 KDa.
Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]

Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]

Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165] - IHC image of Muscarinic Acetylcholine Receptor M3/CHRM3 antibody diluted at 1:100 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CHRM3

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.

Long Name

Cholinergic Receptor Muscarinic 3

Alternate Names

HM3, mAChR M3

Gene Symbol

CHRM3

Additional CHRM3 Products

Product Documents for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.