Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15165
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2D4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3/CHRM3. UniProt ID (P20309).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Immunohistochemistry: Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 mAb (2D4) [NBP3-15165]
Immunohistochemistry: Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 mAb (2D4) [NBP3-15165] - Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody diluted at 1:300 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.
Immunohistochemistry: Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 mAb (2D4) [NBP3-15165]
Immunohistochemistry: Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 mAb (2D4) [NBP3-15165] - Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody diluted at 1:300 and staining in paraffin-embedded human endometrial cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.Applications for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Application
Recommended Usage
Immunohistochemistry
1:200-1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: CHRM3
Long Name
Cholinergic Receptor Muscarinic 3
Alternate Names
HM3, mAChR M3
Gene Symbol
CHRM3
Additional CHRM3 Products
Product Documents for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Product Specific Notices for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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