Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15165
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2D4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Immunogen
A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3/CHRM3. UniProt ID (P20309).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
![Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]](https://resources.bio-techne.com/images/products/Muscarinic-Acetylcholine-Receptor-M3-CHRM3-Antibody-2D4-Western-Blot-NBP3-15165-img0002.jpg "Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]")
Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]
Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165] - Positive WB detected in:PC3 whole cell lysate. All lanes: Muscarinic Acetylcholine Receptor M3/CHRM3 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 67 KDa. Observed band size: 67 KDa.![Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]](https://resources.bio-techne.com/images/products/Muscarinic-Acetylcholine-Receptor-M3-CHRM3-Antibody-2D4-Immunohistochemistry-NBP3-15165-img0001.jpg "Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]")
Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165]
Immunohistochemistry: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) [NBP3-15165] - IHC image of Muscarinic Acetylcholine Receptor M3/CHRM3 antibody diluted at 1:100 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CHRM3
Long Name
Cholinergic Receptor Muscarinic 3
Alternate Names
HM3, mAChR M3
Gene Symbol
CHRM3
Additional CHRM3 Products
Product Documents for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
Product Specific Notices for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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