Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20013

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-20013-100ul
NBP3-20013-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-6B4

Concentration

0.3 mg/ml

View all Muscle Phosphofructokinase/PFKM Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human Muscle Phosphofructokinase/PFKM/PFK-1 (Uniprot # P08237)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4)

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Western blot detection of Muscle Phosphofructokinase/PFKM/PFK-1 in C6, 3T3, Hela cell lysates using NBP3-20013 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.
Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013]

Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013]

Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4) [NBP3-20013] - Immunofluorescence of Muscle Phosphofructokinase/PFKM/PFK-1 (green) in Hela cells using NBP3-20013 at dilution 1/200, and DAPI (blue)

Applications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Muscle Phosphofructokinase/PFKM

Phosphofructokinases (PFK) are regulatory glycolytic enzymes that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Human PFK-1 is tetrameric and isoenzymes include PFK-1 muscle (PFKM, PFK-A), PFK-1 liver (PFKL, PFK-B) and PFK-1 platelet (PFKP, PFK-C, PFKF). PFK-1 is inhibited by ATP and citrate (from the tricarboxylic acid cycle). PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose 2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate and contains fructose 2,6-biphosphatase activity that catalyzes the degradation of fructose 2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (S08-6B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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