Muscle Phosphofructokinase/PFKM/PFK-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44228
Key Product Details
Species Reactivity
Rabbit
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length native Muscle Phosphofructokinase/PFKM/PFK-1 purified from rabbit muscle (Uniprot: P00511)
Reactivity Notes
Cross reactivity against Muscle Phosphofructokinase/PFKM/PFK-1 from other sources is expected based on high degrees of sequence homology for muscle derived F6PK. Partial reactivity may occur against F6PK isolated from liver.
Specificity
Partial reactivity may occur against F6PK isolated from liver.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit muscle]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody
![Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228]](https://resources.bio-techne.com/images/products/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Western-Blot-NBP2-44228-img0003.jpg "Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228]")
Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228]
Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Analysis demonstrates the labeling specificity of the antibodies used for Tissue lysate from hog carotid arteries was analyzed by SDS-PAGE. Nitrocellulose membranes were probed with the corresponding antibodies at the same concentrations used for immunofluorescence labeling. 0.05 (PFK) and 0.2 mg (CAV-1) was loaded into lanes 1 and 2, respectively. Western blot development resulted in identification of the proteins of interest at the expected sizes, demonstrating the labeling specificity of the antibodies. Molecular weights are given. Used with permission from Vallejo and Hardin (2004).![Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228]](https://resources.bio-techne.com/images/products/Muscle-Phosphofructokinase-PFKM-PFK-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-44228-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228]")
Immunocytochemistry/ Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228]
Immunocytochemistry/Immunofluorescence: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody [NBP2-44228] - Cultures of A7r5 VSM cells (rat aorta) were labeled with antibody and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).
Muscle Phosphofructokinase/PFKM/PFK-1 Antibody
Confocal microscopy images using anti-F6PK MAGP-2 antibody shows specific staining. Cultures of A7r5 VSM cells (rat aorta) were labeled with anti-F6PK (PFK) at 1:200 and anti-caveolin (CAV)-1. Images from labeled A7r5 cells were acquired separately for PFK fluorescence (green) and CAV-1 fluorescence (red) from each cell. Used with permission from Vallejo and Hardin (2004).Applications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody
Application
Recommended Usage
ELISA
1:1000-1:5000
Immunocytochemistry/ Immunofluorescence
1:200-1:2000
Western Blot
1:500-1:2000
Application Notes
This product has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Muscle Phosphofructokinase/PFKM
Long Name
Phosphofructokinase, Muscle
Alternate Names
GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase
Gene Symbol
PFKM
UniProt
Additional Muscle Phosphofructokinase/PFKM Products
Product Documents for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody
Product Specific Notices for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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