Muscleblind-like 1 Antibody (1H1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50062

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse (Negative), Rat (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H1

Concentration

1 mg/ml

View all Muscleblind-like 1 Antibodies »

Product Summary for Muscleblind-like 1 Antibody (1H1)

Immunogen

Full-length recombinant human muscleblind-like 1 protein expressed in and purified from E. coli. [UniProt# Q9NR56]

Reactivity Notes

No reactivity with rat or mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Muscleblind-like 1 Antibody (1H1)

Western Blot: Muscleblind-like 1 Antibody (1H1) [NBP2-50062]

Western Blot: Muscleblind-like 1 Antibody (1H1) [NBP2-50062]

Western Blot: Muscleblind-like 1 Antibody (1H1) [NBP2-50062] - Analysis of whole cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to MBNL1, NBP2-50062, dilution 1:1,000 in green: [1] protein standard (red), [2] HEK293 cytosol, [3] HEK293 nuclear fraction, [4] NIH-3T3 cytosol, [5] NIH-3T3 nuclear fraction, [6] HeLa cytosol, and [7] HeLa nuclear fraction. The strong band at the 40kDa corresponds to the MBNL1 protein that is predominantly detected in the nuclear enriched fractions. This antibody binds exclusively to the human protein and does not bind the rodent homologue.
Immunocytochemistry/ Immunofluorescence: Muscleblind-like 1 Antibody (1H1) [NBP2-50062]

Immunocytochemistry/ Immunofluorescence: Muscleblind-like 1 Antibody (1H1) [NBP2-50062]

Immunocytochemistry/Immunofluorescence: Muscleblind-like 1 Antibody (1H1) [NBP2-50062] - Analysis of HeLa cells stained with mouse mAb to MBNL1, NBP2-50062, dilution 1:1,000 in green and chicken pAb to HSP60, dilution 1:5,000, in red. NBP2-50062 antibody specifically labels nuclei.

Applications for Muscleblind-like 1 Antibody (1H1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:1000 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Muscleblind-like 1

Several important human diseases are associated with expansion of polynucleotide sequences, in most cases trinucleotide repeats. Myotonic dystrophy (DM1) is one of these diseases, and is associated with increases in the number of CTG repeats in the UTR of the gene encoding myotonin (a.k.a. DM Kinase, DMK or myotonin-Protein Kinase), a ser/thr kinase expressed specifically in muscle. Mammalian genomes contain three muscleblind-like proteins, generally referred to as MBNL1, MBNL2 and MBNL3, and all three were found to associate with long double stranded CUG RNA repeats, thus, being triplet repeat expansion dsRNA-binding proteins.

Alternate Names

DKFZp686P06174, EXP40, EXP42, EXPKIAA0428EXP35, MBNL, muscleblind (Drosophila)-like, muscleblind-like (Drosophila), muscleblind-like protein 1, Triplet-expansion RNA-binding protein

Gene Symbol

MBNL1

UniProt

Q9NR56 (Human)

Additional Muscleblind-like 1 Products

Product Documents for Muscleblind-like 1 Antibody (1H1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Muscleblind-like 1 Antibody (1H1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.