Muscleblind-like 1 Antibody (JE38-34)

Western Blot: Muscleblind-like 1 Antibody (JE38-34) [NBP3-32573]

Western Blot: Muscleblind-like 1 Antibody (JE38-34) [NBP3-32573] - Western blot analysis of Muscleblind-like 1 on different lysates with Rabbit anti-Muscleblind-like 1 antibody (NBP3-32573) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate
Lane 2: K-562 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: U-2 OS cell lysate
Lane 5: A549 cell lysate
Lane 6: HeLa cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 42 kDa
Observed band size: 37/42 kDa

Exposure time: 2 minutes 33 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32573) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.