MxA/Mx1 Antibody (4812)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43704
![Western Blot: MxA/Mx1 Antibody (4812) [NBP2-43704]](https://resources.bio-techne.com/images/products/MxA-Mx1-Antibody-4812-Western-Blot-NBP2-43704-img0001.jpg "Western Blot: MxA/Mx1 Antibody (4812) [NBP2-43704]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque)
Predicted:
Chimpanzee (99%), Rhesus Macaque (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 4812
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human MxA/Mx1. The exact sequence is proprietary.
Reactivity Notes
Mouse (83%), Dog (85%), Pig (83%), Sheep (80%), Bovine (84%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MxA/Mx1 Antibody (4812)
Western Blot: MxA/Mx1 Antibody (4812) [NBP2-43704]
Western Blot: MxA/Mx1 Antibody (4812) [NBP2-43704] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human MX1-transfected 293T cells 7.5 % SDS-PAGE MX1 antibody [4812] dilution: 1:10000.![Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (4812) [NBP2-43704]](https://resources.bio-techne.com/images/products/MxA-Mx1-Antibody-4812-Immunocytochemistry-Immunofluorescence-NBP2-43704-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (4812) [NBP2-43704]")
Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (4812) [NBP2-43704]
Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody (4812) [NBP2-43704] - HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min. Green: MX1 protein stained by MX1 antibody [4812] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Western Blot: MxA/Mx1 Antibody (4812) [NBP2-43704] -
Western Blot: MxA/Mx1 Antibody (4812) [NBP2-43704] -Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [4812] diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.Applications for MxA/Mx1 Antibody (4812)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MxA/Mx1
Long Name
Interferon-regulated Resistance GTP-binding protein MxA
Alternate Names
IFI-78K, IFI78, Mx1
Gene Symbol
MX1
Additional MxA/Mx1 Products
Product Documents for MxA/Mx1 Antibody (4812)
Product Specific Notices for MxA/Mx1 Antibody (4812)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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