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Mxi1 Antibody (1B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00004601-M11

Novus Biologicals, part of Bio-Techne
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H00004601-M11

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MXI1 (AAH12907, 1 a.a. ~ 182 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPSPRLQHSKPPRRLSRAQKHSSGSSNTSTANRRAHLRLCLERLKVLIPLGPDCTRHTTLGLLNKAKAHIKKLEEAERKSQHQLENLEREQRFLKWRLEQLQGPQEMERIRMDSIGSTISSDRSDSEREEIEVDVESTEFSHGEVDNISTTSISDIDDHSSLPSIGSDEGYSSASVKLSFTS

Specificity

MXI1 - MAX interactor 1 (1B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Mxi1 Antibody (1B10)

Immunocytochemistry/ Immunofluorescence: Mxi1 Antibody (1B10) [H00004601-M11]

Immunocytochemistry/ Immunofluorescence: Mxi1 Antibody (1B10) [H00004601-M11]

Immunocytochemistry/Immunofluorescence: Mxi1 Antibody (1B10) [H00004601-M11] - Analysis of monoclonal antibody to MXI1 on U-2 OS cell. Antibody concentration 10 ug/ml
Immunocytochemistry/ Immunofluorescence: Mxi1 Antibody (1B10) [H00004601-M11]

Immunocytochemistry/ Immunofluorescence: Mxi1 Antibody (1B10) [H00004601-M11]

Immunocytochemistry/Immunofluorescence: Mxi1 Antibody (1B10) [H00004601-M11] - Analysis of monoclonal antibody to MXI1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Mxi1 Antibody (1B10) [H00004601-M11] - Detection limit for recombinant GST tagged MXI1 is 0.03 ng/ml as a capture antibody.

Applications for Mxi1 Antibody (1B10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Mxi1

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. [provided by RefSeq]

Long Name

MAX Interacting Protein 1

Alternate Names

MAD2, MXD2, MXI, MXI-WR

Entrez Gene IDs

4601 (Human)

Gene Symbol

MXI1

Additional Mxi1 Products

Product Documents for Mxi1 Antibody (1B10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mxi1 Antibody (1B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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