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Mxi1 Antibody (PCRP-MXI1-1A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07374

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-07374-100ug
NBP3-07374-20ug

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MXI1-1A3

Concentration

0.2 mg/ml

Product Summary for Mxi1 Antibody (PCRP-MXI1-1A3)

Immunogen

Recombinant full-length human Mxi1 protein (Uniprot: P50539)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08353)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Mxi1 Antibody (PCRP-MXI1-1A3)

Immunocytochemistry/ Immunofluorescence: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374]

Immunocytochemistry/ Immunofluorescence: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374]

Immunocytochemistry/Immunofluorescence: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374] - Immunofluorescence analysis of PFA-fixed HeLa cells. Mxi1 Mouse Monoclonal Antibody (PCRP-Mxi1-1A3)
Flow Cytometry: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374]

Flow Cytometry: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374]

Flow Cytometry: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374] - Flow Cytometric Analysis of PFA-fixed HeLa cells. Mxi1 Mouse Monoclonal Antibody (PCRP-Mxi1-1A3) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374]

Protein Array: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374]

Protein Array: Mxi1 Antibody (PCRP-MXI1-1A3) [NBP3-07374] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Mxi1 Mouse Monoclonal Antibody (PCRP-Mxi1-1A3).

Applications for Mxi1 Antibody (PCRP-MXI1-1A3)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Mxi1

Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.

Long Name

MAX Interacting Protein 1

Alternate Names

MAD2, MXD2, MXI, MXI-WR

Gene Symbol

MXI1

UniProt

Additional Mxi1 Products

Product Documents for Mxi1 Antibody (PCRP-MXI1-1A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mxi1 Antibody (PCRP-MXI1-1A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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