MYH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87743

Novus Biologicals, part of Bio-Techne
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NBP1-87743
NBP1-87743-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MYH Antibodies »

Product Summary for MYH Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYH Antibody

Western Blot: MYH Antibody [NBP1-87743]

Western Blot: MYH Antibody [NBP1-87743]

Western Blot: MYH Antibody [NBP1-87743] - Analysis in control (vector only transfected HEK293T lysate) and MUTYH over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MYH Antibody [NBP1-87743]

Immunocytochemistry/ Immunofluorescence: MYH Antibody [NBP1-87743]

Immunocytochemistry/Immunofluorescence: MYH Antibody [NBP1-87743] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Immunohistochemistry-Paraffin: MYH Antibody [NBP1-87743]

Immunohistochemistry-Paraffin: MYH Antibody [NBP1-87743]

Immunohistochemistry-Paraffin: MYH Antibody [NBP1-87743] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Applications for MYH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYH

MYH encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternate Names

EC 3.2.2, EC 3.2.2.-, EC 3.2.2.31, hMYH, MGC4416, MutY homolog, MUTYH, Muyth, MYH, MYHA/G-specific adenine DNA glycosylase

Gene Symbol

MUTYH

Additional MYH Products

Product Documents for MYH Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MYH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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