MYL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86279

Novus Biologicals, part of Bio-Techne
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NBP1-86279
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MYL5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NAFKMLDPDGKGKINKEYIKRLLMSQADKMTAEEVDQMFQFASIDVAGNLDYKALSYVI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYL5 Antibody

Western Blot: MYL5 Antibody [NBP1-86279]

Western Blot: MYL5 Antibody [NBP1-86279]

Western Blot: MYL5 Antibody [NBP1-86279] - Analysis in control (vector only transfected HEK293T lysate) and MYL5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MYL5 Antibody [NBP1-86279]

Immunocytochemistry/ Immunofluorescence: MYL5 Antibody [NBP1-86279]

Immunocytochemistry/Immunofluorescence: MYL5 Antibody [NBP1-86279] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279]

Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279]

Immunohistochemistry-Paraffin: MYL5 Antibody [NBP1-86279] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Applications for MYL5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYL5

MYL5 encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene product, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.

Alternate Names

light polypeptide 5, regulatory, MYLC2, myLC-2, myosin light chain 5, Myosin regulatory light chain 5, myosin, light chain 5, regulatory, Superfast myosin regulatory light chain 2

Gene Symbol

MYL5

Additional MYL5 Products

Product Documents for MYL5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MYL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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