MYL9 Antibody (PSH02-07)

Western Blot: MYL9 Antibody (PSH02-07) [NBP3-32627]

Western Blot: MYL9 Antibody (PSH02-07) [NBP3-32627] - Western blot analysis of MYL9 on different lysates with Rabbit anti-MYL9 antibody (NBP3-32627) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (20 ug/Lane)
Lane 2: MDA-MB-231 cell lysate (20 ug/Lane)
Lane 3: NIH/3T3 cell lysate (20 ug/Lane)
Lane 4: C2C12 cell lysate (20 ug/Lane)
Lane 5: C6 cell lysate (20 ug/Lane)

Predicted band size: 20 kDa
Observed band size: 18 kDa

Exposure time: 2 minutes 45 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32627) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: MYL9 Antibody (PSH02-07) [NBP3-32627]

Flow Cytometry: MYL9 Antibody (PSH02-07) [NBP3-32627] - Flow cytometric analysis of A431 cells labeling MYL9.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32627, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).