MyoD Antibody (SPM427) [Janelia Fluor® 549]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34772JF549

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Chicken

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Janelia Fluor 549

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM427

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MyoD Antibodies »

Product Specifications

Immunogen

Recombinant MyoD of Mouse origin with an epitope mapping to amino acids 180-189. (Uniprot: P15172)

Epitope

aa 180-189

Localization

Nuclear. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Specificity

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this monoclonal antibody maps between amino acid 180-189 in the C-terminal of Mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms sarcomas and alveolar soft part sarcomas.

Marker

Rhabdomyosarcoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for MyoD Antibody (SPM427) [Janelia Fluor® 549]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MyoD

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

Long Name

Myoblast Determination Protein 1

Alternate Names

bHLHc1, MYF3, MYOD1, PUM

Gene Symbol

MYOD1

Additional MyoD Products

Product Documents for MyoD Antibody (SPM427) [Janelia Fluor® 549]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MyoD Antibody (SPM427) [Janelia Fluor® 549]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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