Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Myoferlin Antibodies »

Product Summary for Myoferlin Antibody

Immunogen

The immunogen this antibody was made to, maps to a region between residue 700 to 750 of human Myoferlin using the numbering given in entry NP_038479.1 (GeneID 26509).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myoferlin Antibody

Western Blot: Myoferlin Antibody [NBP2-32264]

Western Blot: Myoferlin Antibody [NBP2-32264]

Western Blot: Myoferlin Antibody [NBP2-32264] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Myoferlin antibody NBP2-32264 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Myoferlin Antibody [NBP2-32264]

Immunoprecipitation: Myoferlin Antibody [NBP2-32264]

Immunoprecipitation: Myoferlin Antibody [NBP2-32264] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-Myoferlin antibody NBP2-32264 used for IP at 6 ug per reaction. Myoferlin was also immunoprecipitated by rabbit anti-Myoferlin antibody BL16508. For blotting immunoprecipitated Myoferlin, NBP2-32264 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Myoferlin Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Myoferlin

Myoferlin belongs to the ferlin family and contains five C2 domains. It is a calcium/phospholipid binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. It is also involved in endocytic recycling and implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Myoferlin is expressed in myoblast and endothelial cells (at protein level), highly expressed in cardiac and skeletal muscles, also present in lung and at very low levels in kidney, placenta and brain. There are eight named isoforms.

Alternate Names

fer-1 (C.elegans)-like 3 (myoferlin), FER1L3, fer-1-like 3, myoferlin, fer-1-like 3, myoferlin (C. elegans), Fer-1-like protein 3, FLJ36571, FLJ90777, KIAA1207, myoferlin

Gene Symbol

MYOF

UniProt

NP_038479.1 (Human)

Additional Myoferlin Products

Product Documents for Myoferlin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myoferlin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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