Myosin 1B Antibody

Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739]

Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.

Western Blot: Myosin 1B Antibody [NBP1-87739]

Western Blot: Myosin 1B Antibody [NBP1-87739] - Lysates of mouse whole brain in different developmental stages. Antibody at 1:1000. Molecular weight of the protein is around 130-140 KDa. Image submitted by a verified customer review

Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739]

Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human liver shows moderate membranous positivity in hepatocytes.

Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739]

Immunohistochemistry-Paraffin: Myosin 1B Antibody [NBP1-87739] - Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.

Western Blot: Myosin 1B Antibody [NBP1-87739] -

Western Blot: Myosin 1B Antibody [NBP1-87739] - Expression & localization of Myo1b & Myo1c in ATII cells. (A) Semi-quantitative RT-PCR showed that Myo1b & Myo1c are among the highest expressed Myo1 isoforms in ATII cells. Data (mean±s.e.m.) obtained from three cell isolations & three experiments per isolation are shown relative to the expression of the housekeeping gene HMBS. Day 0, freshly isolated cells; Day 2, after 2 days in culture. (B) Western blot of Myo1b & Myo1c from freshly isolated ATII cells & ATII cells after 2 days of culture (0 & 2, respectively) to demonstrate expression of Myo1b & Myo1c in ATII cells. Ponceau S staining was used to control for equal protein loading (M, marker). (C) Colocalization of anti Myo1b & Myo1c staining with fused secretory vesicles marked by ABCa3 & phalloidin immunostaining. The ABCa3 transporter is a marker for secretory vesicle membrane in ATII cells. After ATP stimulation, some vesicles fused with the plasma membrane & acquired an actin coat, which was labeled by phalloidin staining (arrow). Scale bars: 10 µm. Insets show the enlarged view of the fused lamellar bodies. Scale bars: 1 µm. Image collected & cropped by CiteAb from the following publication (https://journals.biologists.com/jcs/article/doi/10.1242/jcs.181313/260175/The-role-of-myosin-1c-and-myosin-1b-for-surfactant), licensed under a CC-BY license. Not internally tested by Novus Biologicals.