Myosin 5a Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49858
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ETKQLELDLN corresponding to internal region according to NP_001135967.1.
Epitope
ETKQLELDLN - internal region
Specificity
This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Myosin 5a Antibody
![Western Blot: Myosin 5a Antibody [NBP1-49858]](https://resources.bio-techne.com/images/products/Myosin-5a-Antibody-Western-Blot-NBP1-49858-img0001.jpg "Western Blot: Myosin 5a Antibody [NBP1-49858]")
Western Blot: Myosin 5a Antibody [NBP1-49858]
Western Blot: Myosin 5a Antibody [NBP1-49858] - Analysis of Myosin 5a in Jurkat lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-49858]](https://resources.bio-techne.com/images/products/Myosin-5a-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49858-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-49858]")
Immunocytochemistry/ Immunofluorescence: Myosin 5a Antibody [NBP1-49858]
Immunocytochemistry/Immunofluorescence: Myosin 5a Antibody [NBP1-49858] - (1ug/ml) staining of Mouse Skeletal Muscle (first panel, and in green in third panel). Alpha-bungaratoxin staining in middle panel and in red in third panel. Detected by Fluorescence. Data kindly provided by Dr. Rudiger Rudolf, Karlsruhe, GermanyApplications for Myosin 5a Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1 - 3 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
1 - 3 ug/ml
Application Notes
IF: Transverse sections of mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodiesscientific literature (PMID: 20133847). WB: Approx. 200 kDa band observed in Jurkat lysates (calculated MW of 215 kDa band according to NP_000250.3).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Myosin 5a
Alternate Names
Dilute myosin heavy chain, non-muscle, GS1myosin, heavy polypeptide kinase, MYH12myosin-12, MYO5, Myosin heavy chain 12, myosin V, myosin VA (heavy chain 12, myoxin), myosin VA (heavy polypeptide 12, myoxin), Myosin-12, myosin-Va, Myoxin, MYR12
Entrez Gene IDs
4644 (Human)
Gene Symbol
MYO5A
UniProt
Additional Myosin 5a Products
Product Documents for Myosin 5a Antibody
Product Specific Notices for Myosin 5a Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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