Myosin Heavy Chain 1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-95223
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3). MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
223 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Myosin Heavy Chain 1 Antibody - Azide and BSA Free
![Western Blot: Myosin Heavy Chain 1 AntibodyAzide and BSA Free [NBP2-95223]](https://resources.bio-techne.com/images/products/Myosin-Heavy-Chain-1-Antibody-Western-Blot-NBP2-95223-img0007.jpg "Western Blot: Myosin Heavy Chain 1 AntibodyAzide and BSA Free [NBP2-95223]")
Western Blot: Myosin Heavy Chain 1 AntibodyAzide and BSA Free [NBP2-95223]
Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] - Analysis of extracts of various cell lines, using MYH1 antibody at 1:410 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10038 dilution.Lysates/proteins: 63ug per lane. Blocking buffer: 41% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] -
Western Blot: Myosin Heavy Chain 1 Antibody [NBP2-95223] -analysis of lysates from Mouse skeletal muscle using MYH1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time:3s.
Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody [NBP2-95223] -
Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody [NBP2-95223] -Immunofluorescence analysis of paraffin-embedded mouse heart using MYH1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for Myosin Heavy Chain 1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:100 - 1:500
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Myosin Heavy Chain 1/MYH1
Long Name
Myosin Heavy Chain 1
Alternate Names
MGC133384, MYH1, MyHC-2x, MyHC-IIx/d, Myosin-1
Gene Symbol
MYH1
Additional Myosin Heavy Chain 1/MYH1 Products
Product Documents for Myosin Heavy Chain 1 Antibody - Azide and BSA Free
Product Specific Notices for Myosin Heavy Chain 1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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