Myosin heavy chain 11 Antibody (CL14617)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26874

Novus Biologicals, part of Bio-Techne
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NBP3-26874-25ul
NBP3-26874-100ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL14617

Concentration

1 mg/ml

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Product Summary for Myosin heavy chain 11 Antibody (CL14617)

Immunogen

This antibody was developed against a Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence of P35749. Actual immunogen sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Myosin heavy chain 11 Antibody (CL14617)

Myosin heavy chain 11 Antibody (CL14617)

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (CL14617) [NBP3-26874]

Analysis in human smooth muscle and liver tissues. Corresponding RNA-seq data are presented for the same tissues.
Myosin heavy chain 11 Antibody (CL14617)

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (CL14617) [NBP3-26874]

Staining of human liver shows no positivity in hepatocytes as expected.
Myosin heavy chain 11 Antibody (CL14617)

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (CL14617) [NBP3-26874]

Staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
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Applications for Myosin heavy chain 11 Antibody (CL14617)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin heavy chain 11

The protein encoded by the MYH11 gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. MYH11 functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

AAT4, FAA4, MYH11, MYH11 myosin, heavy chain 11, smooth muscle, SMHC, SMMHC, Smooth muscle myosin heavy chain

Gene Symbol

MYH11

Additional Myosin heavy chain 11 Products

Product Documents for Myosin heavy chain 11 Antibody (CL14617)

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Product Specific Notices for Myosin heavy chain 11 Antibody (CL14617)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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