Myosin heavy chain 11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87025

Novus Biologicals, part of Bio-Techne
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NBP1-87025
NBP1-87025-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Myosin heavy chain 11 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VTVLKKALDEETRSHEAQVQEMRQKHAQAVEELTEQLEQFKRAKANLDKNKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEAQVQELQSKCSDGE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin heavy chain 11 Antibody

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025]

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025]

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Analysis in human prostate and skeletal muscle tissues. Corresponding MYH11 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody [NBP1-87025]

Immunocytochemistry/ Immunofluorescence: Myosin heavy chain 11 Antibody [NBP1-87025]

Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human cell line U-2 OS shows positivity in nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025]

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025]

Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
View 3 more images

Applications for Myosin heavy chain 11 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin heavy chain 11

The protein encoded by the MYH11 gene is a smooth muscle myosin belonging to the myosin heavy chain family. The gene product is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. MYH11 functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP. The gene encoding a human ortholog of rat NUDE1 is transcribed from the reverse strand of this gene, and its 3' end overlaps with that of the latter. The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Alternative splicing generates isoforms that are differentially expressed, with ratios changing during muscle cell maturation. Alternatively spliced transcript variants encoding different isoforms have been identified.

Alternate Names

AAT4, FAA4, MYH11, MYH11 myosin, heavy chain 11, smooth muscle, SMHC, SMMHC, Smooth muscle myosin heavy chain

Gene Symbol

MYH11

Additional Myosin heavy chain 11 Products

Product Documents for Myosin heavy chain 11 Antibody

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Certificate of Analysis

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Product Specific Notices for Myosin heavy chain 11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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