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Myosin light chain 3 Antibody (4C2)

Novus Biologicals, part of Bio-Techne | Catalog # H00004634-M12

Novus Biologicals, part of Bio-Techne
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H00004634-M12

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MYL3 (AAH09790, 1 a.a. ~ 195 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS

Specificity

MYL3 - myosin, light polypeptide 3, alkali; ventricular, skeletal, slow (4C2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Myosin light chain 3 Antibody (4C2)

Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12]

Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12]

Western Blot: Myosin light chain 3 Antibody (4C2) [H00004634-M12] - Analysis of MYL3 expression in transfected 293T cell line by MYL3 monoclonal antibody (M12), clone 4C2. Lane 1: MYL3 transfected lysatE (21.9 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Myosin light chain 3 Antibody (4C2) [H00004634-M12] - Detection limit for recombinant GST tagged MYL3 is approximately 3ng/ml as a capture antibody.

Applications for Myosin light chain 3 Antibody (4C2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myosin light chain 3

MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq]

Alternate Names

Cardiac myosin light chain 1, CMH8Ventricular/slow twitch myosin alkali light chain, CMLC1, light polypeptide 3, alkali; ventricular, skeletal, slow, MLC1SBMyosin light chain 1, slow-twitch muscle B/ventricular isoform, MLC1V, myosin light chain 3, myosin, light chain 3, alkali; ventricular, skeletal, slow, VLC1

Entrez Gene IDs

4634 (Human)

Gene Symbol

MYL3

Additional Myosin light chain 3 Products

Product Documents for Myosin light chain 3 Antibody (4C2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myosin light chain 3 Antibody (4C2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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