Myosin Phosphatase [p Thr853] Antibody (SA19)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29680

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-29680-0.025ml
NBP2-29680
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Chicken

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SA19

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myosin Phosphatase Antibodies »

Product Specifications

Immunogen

KLH-conjugated, synthetic peptide containing the sequence.RS[pT]GV., in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Reactivity Notes

Chicken, Human

Modification

p Thr853

Specificity

MYPT1 phosphorylated on Thr850doublet at 130kDa weight may be detected.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Relevant Entrez Gene Numbers: NM_002480

Scientific Data Images for Myosin Phosphatase [p Thr853] Antibody (SA19)

Western Blot: Myosin Phosphatase [p Thr853] Antibody (SA19) [NBP2-29680] - Untreated HEK293 cell lysate (lane 1) and HEK293 cell lysate phosphorylated by ROKalpha /ROCKII (lane 2) were probed with anti-phospho-MyPT1 (Thr850), clone SA19 (1:1000 dilution).

Applications for Myosin Phosphatase [p Thr853] Antibody (SA19)

Application
Recommended Usage

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

(0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 30% glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myosin Phosphatase

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Alternate Names

MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit

Gene Symbol

PPP1R12A

UniProt

O14974 (Human)

Additional Myosin Phosphatase Products

Product Documents for Myosin Phosphatase [p Thr853] Antibody (SA19)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Myosin Phosphatase [p Thr853] Antibody (SA19)

Relevant Entrez Gene Numbers: NM_002480

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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