Myosin Phosphatase [p Thr850, p Thr853] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13185
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to aa845-855, C-EKRRS[pT]GVSFW of chicken MYPT1 with an N-terminal cysteine added for conjugation purposes. Phosphorylation site corresponds to Thr853 in human MYPT1.
Reactivity Notes
Crossreactivity: human (Thr853, O014974), chicken (Thr850, Q90623), mouse (Thr852, Q9DBR7) and rat (Thr855, Q10728).
Modification
p Thr853, p Thr850
Specificity
Recognizes recombinant truncated MYPT1 phosphorylated on Thr853, MW ~80kDa
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Scientific Data Images for Myosin Phosphatase [p Thr850, p Thr853] Antibody
Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: untreated C2C12 lysate| Lane 2: pervanadate treated C2C12 lysate. Resolved by electrophoresis, transferred to nitrocellulose and probed with Myosin Phosphatase [p Thr853] antibody (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Arrow indicates phosphorylated MYPT1 substrate (80kD).
Myosin Phosphatase [p Thr853] Antibody [NBP2-13185] - Lane 1: ROKa/ROCK-II only Lane 2: MYPT1 substrate only Lane 3: MYPT1 substrate posphorylated by ROKa/ROCK-II 3
Applications for Myosin Phosphatase [p Thr850, p Thr853] Antibody
Application
Recommended Usage
Western Blot
0.5-2 ug/ml
Application Notes
Western Blot: Detects MYPT1 by preincubation with ROKa/ROK-II active or C2C12 lysates treated with pervanadate. Optimal dilutions to be determined by the researcher.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Myosin Phosphatase
Alternate Names
MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit
Gene Symbol
PPP1R12A
UniProt
Additional Myosin Phosphatase Products
Product Documents for Myosin Phosphatase [p Thr850, p Thr853] Antibody
Product Specific Notices for Myosin Phosphatase [p Thr850, p Thr853] Antibody
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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