Myosin Phosphatase [p Thr696] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16931

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Myosin Phosphatase Antibodies »

Product Summary for Myosin Phosphatase [p Thr696] Antibody - Azide and BSA Free

Immunogen

A synthetic phosphorylated peptide around T696 of human Myosin Phosphatase (NP_002471.1). RSTQG

Modification

p Thr696

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin Phosphatase [p Thr696] Antibody - Azide and BSA Free

Western Blot: Myosin Phosphatase [p Thr696] AntibodyAzide and BSA Free [NBP3-16931]

Western Blot: Myosin Phosphatase [p Thr696] AntibodyAzide and BSA Free [NBP3-16931]

Western Blot: Myosin Phosphatase Antibody [NBP3-16931] - Western blot analysis of extracts of Jurkat cells, using Myosin Phosphatase Rabbit pAb (NBP3-16931) at 1:1000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Applications for Myosin Phosphatase [p Thr696] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Myosin Phosphatase

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Alternate Names

MBSmyosin phosphatase, target subunit 1, MGC133042, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, MYPT1protein phosphatase 1 regulatory subunit 12A, protein phosphatase 1, regulatory (inhibitor) subunit 12A, Protein phosphatase myosin-binding subunit

Gene Symbol

PPP1R12A

Additional Myosin Phosphatase Products

Product Documents for Myosin Phosphatase [p Thr696] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Myosin Phosphatase [p Thr696] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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