Myosin VIIb Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27882
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
1 mg/ml
Product Specifications
Immunogen
Myosin VIIb antibody was raised against a peptide corresponding to 15 amino acids near the center of human Myosin VIIb. The immunogen is located within amino acids 1100-1150 of Myosin VIIb.
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for Myosin VIIb Antibody
Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] -
Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] - Figure 3 Immunohistochemistry Validation of Myosin VIIb in Human Kidney TissueImmunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Myosin VIIb antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: Myosin VIIb Antibody [NBP3-27882] -
Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: Myosin VIIb Antibody [NBP3-27882] -
Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Applications for Myosin VIIb Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/mL
Western Blot
1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Myosin VIIb
Additional Myosin VIIb Products
Product Documents for Myosin VIIb Antibody
Product Specific Notices for Myosin VIIb Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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