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Myosin VIIb Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27882

Novus Biologicals, part of Bio-Techne
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NBP3-27882

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

1 mg/ml

Product Specifications

Immunogen

Myosin VIIb antibody was raised against a peptide corresponding to 15 amino acids near the center of human Myosin VIIb. The immunogen is located within amino acids 1100-1150 of Myosin VIIb.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for Myosin VIIb Antibody

Myosin VIIb Antibody

Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] -

Immunohistochemistry: Myosin VIIb Antibody [NBP3-27882] - Figure 3 Immunohistochemistry Validation of Myosin VIIb in Human Kidney Tissue
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Myosin VIIb antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Myosin VIIb Antibody

Western Blot: Myosin VIIb Antibody [NBP3-27882] -

Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 2 Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 u of lysates per lane.Antibodies: Myosin VIIb (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Myosin VIIb Antibody

Western Blot: Myosin VIIb Antibody [NBP3-27882] -

Western Blot: Myosin VIIb Antibody [NBP3-27882] - Figure 2 Western Blot Validation in Human and Mouse Cell LinesLoading: 15 u of lysates per lane.Antibodies: Myosin VIIb (2 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Applications for Myosin VIIb Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Western Blot

1 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin VIIb

Myosin VIIb, or MYO7B, consists of a long 2,116 amino acid isoform that is 242 kDa, and is involved in transportation, though more research must be conducted to determine more about the protein. Current research is being conducted on the relationship between Myosin VIIb and several diseases and disorders, including Hepatitis B, kidney disease, and Usher syndrome. Myosin VIIb is not linked to any pathways, but has been found to interact with UBE2A and USH1C.

Alternate Names

DKFZp686A08248, myosin VIIB

Gene Symbol

MYO7B

UniProt

Additional Myosin VIIb Products

Product Documents for Myosin VIIb Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Myosin VIIb Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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