Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all N-Acetyl-D-Glucosamine Kinase/NAGK Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 50 and 100 of human N-acetylglucosamine kinase using the numbering given in entry NP_060037.2 (GeneID 55577).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody [NBP1-42694]

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody [NBP1-42694]

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody [NBP1-42694] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-NAGK antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NAGK was also immunoprecipitated by another rabbit anti-NAGK antibody, which recognizes an upstream epitope.

Applications for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: N-Acetyl-D-Glucosamine Kinase/NAGK

N-acetylglucosamine kinase (NAGK; EC 2.7.1.59) converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. NAGK belongs to the group of N-acetylhexosamine kinases and is a prominent salvage enzyme of amino sugar metabolism in mammals.[supplied by OMIM]

Long Name

N-Acetyl-D-Glucosamine Kinase

Alternate Names

GlcNAc Kinase, GNK, N-Acetylglucosamine Kinase

Entrez Gene IDs

55577 (Human)

Gene Symbol

NAGK

Additional N-Acetyl-D-Glucosamine Kinase/NAGK Products

Product Documents for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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