N-WASP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23275

Novus Biologicals, part of Bio-Techne
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NBP3-23275
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all N-WASP Antibodies »

Product Summary for N-WASP Antibody

Immunogen

A synthetic peptide (coupled to KLH) corresponding to a region near the C-terminus of human WASP. This sequence is highly homologous with a similar region in human N-WASP. [UniProt# O00401]

Specificity

This antibody detects a 63 kDa protein corresponding to the molecular mass of WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to the molecular mass of N-WASP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for N-WASP Antibody

N-WASP Antibody

Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23275] -

Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP3-23275] - Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (NBP3-23275) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.
N-WASP Antibody

Western Blot: N-WASP Antibody [NBP3-23275] -

Western Blot: N-WASP Antibody [NBP3-23275] - Western blot analysis of Jurkat (lane 1), A431 (lane 2), and HeLa (lane 3) cell lysates (20 ug/lane). Blots were probed with rabbit polyclonal anti-WASP/N-WASP (NBP3-23275).

Applications for N-WASP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: N-WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Gene Symbol

WASL

Additional N-WASP Products

Product Documents for N-WASP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for N-WASP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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