N-WASP [p Tyr256] Antibody

Biological Validation

Validated:

Unconjugated

Polyclonal Rabbit IgG

Please see the vial label for concentration. If unlisted please contact technical services.

Phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291. [UniProt# O00401]

p Tyr256

This antibody was cross-adsorbed to phosphotyrosine then affinity purified using phospho-N-WASP (Tyr-256) peptide (without carrier). The antibody detects a 65 kDa* protein corresponding to the molecular mass of phosphorylated N-WASP on SDS-PAGE immunoblots of A431 cells treated with pervanadate. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at higher molecular weights after pervanadate treatment. These bands may be due to low cross-reactivity with phosphotyrosine.

Polyclonal

Rabbit

IgG

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Stable for at least 1 year at -20°C.

Antigen Affinity-purified

PBS, 1 mg/ml BSA and 50% glycerol

0.05% Sodium Azide

Please see the vial label for concentration. If unlisted please contact technical services.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C.