N-WASP [p Ser485, p Ser484] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23284

Novus Biologicals, part of Bio-Techne
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NBP3-23284
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all N-WASP Antibodies »

Product Summary for N-WASP [p Ser485, p Ser484] Antibody

Immunogen

Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484. [UniProt# O00401]

Modification

p Ser485, p Ser484

Specificity

This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide then affinity purified using phospho-N-WASP (S484/S485) peptide. The antibody detects a 63 kDa protein corresponding to the molecular mass of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for N-WASP [p Ser485, p Ser484] Antibody

N-WASP [p Ser485, p Ser484] Antibody

Immunocytochemistry/Immunofluorescence: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] -

Immunocytochemistry/Immunofluorescence: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Immunocytochemical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.
N-WASP [p Ser485, p Ser484] Antibody

Western Blot: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] -

Western Blot: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

Applications for N-WASP [p Ser485, p Ser484] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: N-WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Gene Symbol

WASL

Additional N-WASP Products

Product Documents for N-WASP [p Ser485, p Ser484] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for N-WASP [p Ser485, p Ser484] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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