NAB1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71838
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Golden Syrian Hamster (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Simple Western, Western Blot
Cited:
Block/Neutralize, Chemotaxis, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 460 and 487 of human NGFI-A Binding Protein 1 using the numbering given in entry NP_005957.2 (GeneID 4664).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NAB1 Antibody
![Western Blot: NAB1 Antibody [NBP1-71838]](https://resources.bio-techne.com/images/products/NAB1-Antibody-Western-Blot-NBP1-71838-img0003.jpg "Western Blot: NAB1 Antibody [NBP1-71838]")
Western Blot: NAB1 Antibody [NBP1-71838]
Western Blot: NAB1 Antibody [NBP1-71838] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T, 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NAB1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB1 was also immunoprecipitated by rabbit anti-NAB1 antibody NBP1-71837, which recognizes an upstream epitope.![Simple Western: NAB1 Antibody [NBP1-71838]](https://resources.bio-techne.com/images/products/NAB1-Antibody-Simple-Western-NBP1-71838-img0002.jpg "Simple Western: NAB1 Antibody [NBP1-71838]")
Simple Western: NAB1 Antibody [NBP1-71838]
Simple Western: NAB1 Antibody [NBP1-71838] - Simple Western lane view shows a specific band for NAB1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for NAB1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Reported in scientific literature (PMID 23935197)
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NAB1
Alternate Names
EGR1 binding protein 1, EGR-1-binding protein 1, NGFI-A binding protein 1 (EGR1 binding protein 1), NGFI-A-binding protein 1, Transcriptional regulatory protein p54
Gene Symbol
NAB1
UniProt
Additional NAB1 Products
Product Documents for NAB1 Antibody
Product Specific Notices for NAB1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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