NAB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86163

Novus Biologicals, part of Bio-Techne
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NBP1-86163
NBP1-86163-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NAB1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MIGHIFEMNDDDPHKEEEIRKYSAIYGRFDSKRKDGKHLTLHELTVNEAAAQLCVKDNALLTRRDELFALARQISREVTYKYTYRTTKSKCGERDELSPKRIKVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAB1 Antibody

Western Blot: NAB1 Antibody [NBP1-86163]

Western Blot: NAB1 Antibody [NBP1-86163]

Western Blot: NAB1 Antibody [NBP1-86163] - Analysis in control (vector only transfected HEK293T lysate) and NAB1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: NAB1 Antibody [NBP1-86163]

Immunocytochemistry/ Immunofluorescence: NAB1 Antibody [NBP1-86163]

Immunocytochemistry/Immunofluorescence: NAB1 Antibody [NBP1-86163] - Staining of human cell line U-251 MG shows localization to nucleoplasm, plasma membrane & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NAB1 Antibody [NBP1-86163]

Immunohistochemistry-Paraffin: NAB1 Antibody [NBP1-86163]

Immunohistochemistry-Paraffin: NAB1 Antibody [NBP1-86163] - Staining of human lung shows strong membranous positivity in alveolar cells and distinct staining in vessel wall.

Applications for NAB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NAB1

NAB1 acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2

Alternate Names

EGR1 binding protein 1, EGR-1-binding protein 1, NGFI-A binding protein 1 (EGR1 binding protein 1), NGFI-A-binding protein 1, Transcriptional regulatory protein p54

Gene Symbol

NAB1

Additional NAB1 Products

Product Documents for NAB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NAB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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