Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Nab2 Antibodies »

Product Summary for Nab2 Antibody

Immunogen

The immunogen for this product maps to a region between residue 475 and 525 of human NGFI-A Binding Protein 2 using the numbering given in entry NP_005958.1 (GeneID 4665).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nab2 Antibody

Western Blot: Nab2 Antibody [NBP1-71869]

Western Blot: Nab2 Antibody [NBP1-71869]

Western Blot: Nab2 Antibody [NBP1-71869] - Whole cell lysate from 293T (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-NAB2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. NAB2 was also immunoprecipitated by another rabbit anti-NAB2 antibody which recognizes an upstream epitope.

Applications for Nab2 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Nab2

This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Alternate Names

EGR-1-binding protein 2, MADEREGR1 binding protein 2, Melanoma-associated delayed early response protein, MGC75085, NGFI-A binding protein 2 (EGR1 binding protein 2), NGFI-A-binding protein 2, Protein MADER

Entrez Gene IDs

4665 (Human)

Gene Symbol

NAB2

UniProt

Q15742 (Human)

Additional Nab2 Products

Product Documents for Nab2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nab2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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