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NAC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49361

Novus Biologicals, part of Bio-Techne
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NBP2-49361

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RVVRKSWMPKVKVLKAEDDAYTTFISETGKIEPDMMGVEHGFETASHEGEAGPSAEAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAC1 Antibody

Western Blot: NAC1 Antibody [NBP2-49361]

Western Blot: NAC1 Antibody [NBP2-49361]

Western Blot: NAC1 Antibody [NBP2-49361] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: NAC1 Antibody [NBP2-49361]

Immunocytochemistry/ Immunofluorescence: NAC1 Antibody [NBP2-49361]

Immunocytochemistry/Immunofluorescence: NAC1 Antibody [NBP2-49361] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: NAC1 Antibody [NBP2-49361]

Immunohistochemistry-Paraffin: NAC1 Antibody [NBP2-49361]

Immunohistochemistry-Paraffin: NAC1 Antibody [NBP2-49361] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.

Applications for NAC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NAC1

The BTB/POZ gene family shares a conserved BTB/POZ protein-protein interaction motif at the N terminus that mediates homodimer or heterodimer formation. NAC1, a member of this family, has been found to be overexpressed in ovarian cancer. This protein appears to play a critical role in tumorigenesis and in the growth and survival of tumor cells, and Nac1 antibodies are therefore useful for cancer research.

Long Name

Nucleus Accumbens Associated 1

Alternate Names

BEND8, BTBD14B, BTBD30, NACC1

Gene Symbol

NACC1

Additional NAC1 Products

Product Documents for NAC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NAC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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