Nanos3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-76919

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Nanos3 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of the human Nanos3. The immunogen is located within the last 50 amino acids of Nanos3. Amino Acid Squence: CKLVRPDKAKQDTGHR

Specificity

This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nanos3 Antibody - BSA Free

Western Blot: Nanos3 AntibodyBSA Free [NBP1-76919]

Western Blot: Nanos3 AntibodyBSA Free [NBP1-76919]

Western Blot: Nanos3 Antibody [NBP1-76919] - Human brain tissue lysate with Nanos3 antibody at 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Nanos3 Antibody - BSA Free [NBP1-76919]

Immunocytochemistry/ Immunofluorescence: Nanos3 Antibody - BSA Free [NBP1-76919]

Immunocytochemistry/Immunofluorescence: Nanos3 Antibody [NBP1-76919] - in Human Brain cells.
Immunohistochemistry-Paraffin: Nanos3 Antibody - BSA Free [NBP1-76919]

Immunohistochemistry-Paraffin: Nanos3 Antibody - BSA Free [NBP1-76919]

Immunohistochemistry-Paraffin: Nanos3 Antibody [NBP1-76919] - Human brain tissue with Nanos3 antibody at 2.5 ug/ml.

Applications for Nanos3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanos3

Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In Drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2.

Alternate Names

MGC120114, nanos homolog 3, nanos homolog 3 (Drosophila), NOS-3, NOS3NANOS1L

Entrez Gene IDs

342977 (Human)

Gene Symbol

NANOS3

UniProt

P60323 (Human)

Additional Nanos3 Products

Product Documents for Nanos3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nanos3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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