NAPRT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87243

Novus Biologicals, part of Bio-Techne
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NBP1-87243
NBP1-87243-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NAPRT1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLDTYSVWRSGLPNFLAVALALGELGYRAVGVRLDSGDLLQQAQEIRKVFRAAAAQFQVPWLESVLIVVSNNIDEEALARLAQEGSEVNVIGIGTSV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAPRT1 Antibody

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243]

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243]

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Analysis in human small intestine and skeletal muscle tissues using NBP1-87243 antibody. Corresponding NAPRT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243]

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243]

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP1-87243] - Staining of human colon, kidney, lymph node and urinary bladder using Anti-NAPRT antibody NBP1-87243 (A) shows similar protein distribution across tissues to independent antibody NBP1-87244 (B).
Western Blot: NAPRT1 Antibody [NBP1-87243]

Western Blot: NAPRT1 Antibody [NBP1-87243]

Western Blot: NAPRT1 Antibody [NBP1-87243] - Analysis using Anti-NAPRT antibody NBP1-87243 (A) shows similar pattern to independent antibody NBP1-87244 (B).
View 5 more images

Applications for NAPRT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-P reported in scientific literature (PMID: 24097869). Use in immunoprecipitation reported in scientific literature (PMID:31511522). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NAPRT1

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Alternate Names

EC 2.4.2.11, FHA-HIT-interacting protein, FHIP, NAPRTase, nicotinate phosphoribosyltransferase, nicotinate phosphoribosyltransferase domain containing 1, Nicotinate phosphoribosyltransferase domain-containing protein 1, nicotinic acid phosphoribosyltransferase, PP3856

Entrez Gene IDs

93100 (Human)

Gene Symbol

NAPRT

UniProt

Q6XQN6 (Human)

Additional NAPRT1 Products

Product Documents for NAPRT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NAPRT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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