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NAPRT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19471

Novus Biologicals, part of Bio-Techne
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NBP2-19471

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for NAPRT1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NAPRT1. The exact sequence is proprietary.

Localization

Cytoplasm; cytosol

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NAPRT1 Antibody

Western Blot: NAPRT1 Antibody [NBP2-19471]

Western Blot: NAPRT1 Antibody [NBP2-19471]

Western Blot: NAPRT1 Antibody [NBP2-19471] - A: HeLa.
Immunocytochemistry/ Immunofluorescence: NAPRT1 Antibody [NBP2-19471]

Immunocytochemistry/ Immunofluorescence: NAPRT1 Antibody [NBP2-19471]

Immunocytochemistry/Immunofluorescence: NAPRT1 Antibody [NBP2-19471] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using NAPRT1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP2-19471]

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP2-19471]

Immunohistochemistry-Paraffin: NAPRT1 Antibody [NBP2-19471] - Immunohistochemical analysis of paraffin-embedded Lung ca, using antibody at 1:500 dilution.

Applications for NAPRT1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NAPRT1

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Alternate Names

EC 2.4.2.11, FHA-HIT-interacting protein, FHIP, NAPRTase, nicotinate phosphoribosyltransferase, nicotinate phosphoribosyltransferase domain containing 1, Nicotinate phosphoribosyltransferase domain-containing protein 1, nicotinic acid phosphoribosyltransferase, PP3856

Gene Symbol

NAPRT

Additional NAPRT1 Products

Product Documents for NAPRT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NAPRT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.