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Napsin A Antibody (A9C1-R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32633

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32633

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # A9C1-R

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within human Napsin A aa 51-200 / 420. (Uniprot: O96009)

Localization

Secreted.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Napsin A Antibody (A9C1-R)

Napsin A Antibody (A9C1-R)

Immunohistochemistry: Napsin A Antibody (A9C1-R) [NBP3-32633]

Immunohistochemistry: Napsin A Antibody (A9C1-R) [NBP3-32633] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Napsin A antibody (NBP3-32633) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32633) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for Napsin A Antibody (A9C1-R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Napsin A

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Gene Symbol

NAPSA

Additional Napsin A Products

Product Documents for Napsin A Antibody (A9C1-R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Napsin A Antibody (A9C1-R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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