Napsin A Antibody (NAPSA/1238) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54383

Novus Biologicals, part of Bio-Techne
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NBP2-54383-100ug
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NAPSA/1238

Format

BSA Free

Concentration

1 mg/ml

View all Napsin A Antibodies »

Product Specifications

Immunogen

Recombinant human Napsin A protein fragment (around aa189-299) (exact sequence is proprietary) (Uniprot: O96009)

Localization

Cytoplasmic

Marker

Lung Adenocarcinoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-45244).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Napsin A Antibody (NAPSA/1238) - BSA Free

Western Blot: Napsin A Antibody (NAPSA/1238)BSA Free [NBP2-54383]

Western Blot: Napsin A Antibody (NAPSA/1238)BSA Free [NBP2-54383]

Western Blot: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - K562, HEK293 andA549 Cell Lysates Using Napsin-A Monoclonal Antibody (NAPSA/1238)
Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383]

Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383]

Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1238).
Protein Array: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383]

Protein Array: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383]

Protein Array: Napsin A Antibody (NAPSA/1238) - BSA Free [NBP2-54383] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Napsin A Antibody (NAPSA/1238). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Napsin A Antibody (NAPSA/1238) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1 - 2 ug/ml

Western Blot

1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Napsin A

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Alternate Names

ASP4, KAP, NAPA, NAPSA, Napsin-1, SNAPA, TA01/TA02

Gene Symbol

NAPSA

Additional Napsin A Products

Product Documents for Napsin A Antibody (NAPSA/1238) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Napsin A Antibody (NAPSA/1238) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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