Napsin A Antibody (NAPSA/1239)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45245
![Western Blot: Napsin A Antibody (NAPSA/1239) [NBP2-45245]](https://resources.bio-techne.com/images/products/Napsin-A-Antibody-NAPSA-1239-Western-Blot-NBP2-45245-img0002.jpg "Western Blot: Napsin A Antibody (NAPSA/1239) [NBP2-45245]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # NAPSA/1239
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human Napsin A protein fragment (aa189-299) (exact sequence is proprietary) (Uniprot: O96009)
Localization
Cytoplasmic
Marker
Lung Adenocarcinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-54384)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Napsin A Antibody (NAPSA/1239)
Western Blot: Napsin A Antibody (NAPSA/1239) [NBP2-45245]
Western Blot: Napsin A Antibody (NAPSA/1239) [NBP2-45245] - K562 and HEK293 Cell Lysates using Napsin-A Monoclonal Antibody (NAPSA/1239)![Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1239) [NBP2-45245]](https://resources.bio-techne.com/images/products/Napsin-A-Antibody-NAPSA-1239-Immunohistochemistry-Paraffin-NBP2-45245-img0001.jpg "Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1239) [NBP2-45245]")
Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1239) [NBP2-45245]
Immunohistochemistry-Paraffin: Napsin A Antibody (NAPSA/1239) [NBP2-45245] - Human Lung Adenocarcinoma stained with Napsin-A Monoclonal Antibody (NAPSA/1239).Applications for Napsin A Antibody (NAPSA/1239)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Optimal dilution for a specific application should be determined.
Western Blot: 1-2ug/ml for 60 minutes at RT.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Napsin A
Additional Napsin A Products
Product Documents for Napsin A Antibody (NAPSA/1239)
Product Specific Notices for Napsin A Antibody (NAPSA/1239)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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