NASP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33928

Novus Biologicals, part of Bio-Techne
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NBP2-33928
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NASP Antibodies »

Product Summary for NASP Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KESQRSGNVAELALKATLVESSTSGFTPGGGGSSVSMIASRKPTDGASSSNCVTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVNGGSGDAVPSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NASP Antibody

Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928]

Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928]

Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining in human testis and pancreas tissues using anti-NASP antibody. Corresponding NASP RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928]

Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928]

Immunohistochemistry-Paraffin: NASP Antibody [NBP2-33928] - Staining of human liver, lymph node, pancreas and testis using Anti-NASP antibody NBP2-33928 (A) shows similar protein distribution across tissues to independent antibody NBP2-33910 (B).
Western Blot: NASP Antibody [NBP2-33928]

Western Blot: NASP Antibody [NBP2-33928]

Western Blot: NASP Antibody [NBP2-33928] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-NASP antibody. Remaining relative intensity is presented.
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Applications for NASP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NASP

NASP is required for DNA replication, normal cell cycle progression, and cell proliferation. It forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

Alternate Names

DKFZp547F162, FLB7527, FLJ31599, FLJ35510, histone H1-binding protein, MGC19722, MGC20372, MGC2297, nuclear autoantigenic sperm protein, nuclear autoantigenic sperm protein (histone-binding), PRO1999

Gene Symbol

NASP

UniProt

P49321 (Human)

Additional NASP Products

Product Documents for NASP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NASP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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